Immunogenic detoxified mutant E. coli LT-A-toxin
AssigneeChiron Corporation
InventorsMariagrazia Pizza, Marzia Monica Giuliani, Rino Rappuoli
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Abstract
An immunogenic detoxified protein is provided which comprises the amino acid sequence of subunit A of an E. coli heat labile toxin (LT-A) or a fragment thereof in which at least amino acid Ala-72, numbered relative to SEQ ID NO:1, of the A subunits mutated, preferably by substitution with Arg. The toxoid is useful as vaccine against an enterotoxigenic strain of E. coli and is produced by recombinant DNA means by site-directed mutagenesis. It is also an effective adjuvant.
Claims26
A polynucleotide encoding an immunologically effective detoxified E. coli heat labile toxin (LT-A) polypeptide, wherein the polypeptide comprises SEQ ID NO:1, and further wherein the amino acid residue corresponding to Ala-72 of SEQ ID NO:1 is an arginine residue.
A polynucleotide encoding an immunologically effective detoxified E. coli heat labile toxin (LT-A) polypeptide, wherein the polypeptide comprises SEQ ID NO:2, and further wherein the amino acid residue corresponding to Ala-72 of SEQ ID NO:1 is an arginine residue.
A polynucleotide encoding an immunologically effective detoxified E. coli heat labile toxin (LT-A) polypeptide, wherein the polypeptide comprises SEQ ID NO:3, and further wherein the amino acid residue corresponding to Ala-72 of SEQ ID NO:1 is an arginine residue.
A polynucleotide encoding an immunologically effective detoxified E. coli heat labile toxin (LT-A) polypeptide, wherein the polypeptide comprises SEQ ID NO:4, and further wherein the amino acid residue corresponding to Ala-72 of SEQ ID NO:1 is an arginine residue.
Dependencies are inferred from the claim text.