Humanized antibody specific for surface antigen pre-S1 of HBV and preparation method thereof
AssigneesKorea Institute of Science and Technology, Korea Green Cross Corporation
InventorsHyo Jeong Hong, Chun Jeih Ryu, Hyangsuk Hur
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Abstract
The present invention relates to humanized antibodies specific for HBV surface antigen pre-S1, which show binding affinity similar to mouse monoclonal antibody and which show remarkably reduced immunogenicity since they have less mouse-derived amino acid residues. Thus, the humanized antibodies of the present invention may be useful for the prevention of HBV infection and for the treatment of hepatitis B.
Claims8
A gene encoding a humanized heavy chain which comprises a humanized heavy chain variable region having an amino acid sequence selected from the group consisting of:
- the amino acid sequence of SEQ ID NO:
- 21;
- and the amino acid sequence of SEQ ID NO:
- 21 which is modified by at least one amino acid substitution selected from the group comprising:
- Lys12→Val12, Thr28→Ala28, Thr30→Ser30, Met48→Ile48, Arg67→Lys67, Val68→Ala68, Met70→Leu70, Val79→Ala79, and Tyr95→Phe95.
A humanized antibody specific for HBV surface antigen pre-S1, containing a humanized heavy chin variable region which comprises an amino acid sequence of SEQ ID NO:
- 21.
A composition containing a humanized antibody specific for HBV surface ant pre-S1, wherein the humanized antibody comprises:
- (a) a humanized heavy chain variable region which comprises an amino acid sequence of SEQ ID NO:
- 21;
- or
- (b) a humanized heavy chain variable region which comprises an amino acid sequence of SEQ ID NO:
- 21 which is modified by at least one amino acid substitution selected from the group comprising:
- Lys12→Val12, Thr28→Ala28, Thr30→Ser30, Met48→Ile48, Arg67→Lys67, Val68→Ala68, Met70→Leu70, Val79→Ala79, and Tyr95→Phe95.
Dependencies are inferred from the claim text.