Methods of staining target chromosomal DNA employing high complexity nucleic acid probes

A method of staining target interphase chromosomal DNA comprising:

• (a) providing at least one labeled nucleic acid probe having a complexity greater than about 40 kb which labeled nucleic acid probe comprises fragments which are substantially complementary to unique nucleic acid segments within the chromosomal DNA for which detection is desired, wherein the nucleic acid probe is substantially free of repetitive segments which are complementary to repetitive segments in the target interphase chromosomal material;
• and
• (b) employing said labeled nucleic acid probe and chromosomal DNA in in situ hybridization so that hybridization of unique segments within the labeled nucleic acid probe to the chromosomal DNA is allowed, and hybridized labeled nucleic acid containing unique segments are detected, and wherein the interphase chromosomal DNA is present in a morphologically identifiable cell nucleus during the in situ hybridization.

A method of staining target interphase chromosomal DNA comprising:

• providing at least one labeled nucleic acid probe having a complexity greater than about 40 kb which labeled nucleic acid probe comprises fragments which are substantially complementary to unique nucleic acid segments within the chromosomal DNA for which detection is desired, wherein the nucleic acid probe is substantially free of repetitive segments which are complementary to repetitive segments in the target interphase chromosomal material;
• further comprising
• providing blocking nucleic acid that comprises fragments which are substantially complementary to repetitive segments in the labeled nucleic acid probe and employing said labeled nucleic acid probe, blocking nucleic acid, and chromosomal DNA in in situ hybridization so that hybridization of unique segments within the labeled nucleic acid probe to the chromosomal DNA is allowed, labeled repetitive segments, if present, are substantially blocked from binding to the chromosomal DNA, and hybridized labeled nucleic acid containing unique segments are detected, and wherein the interphase chromosomal DNA is present in a morphologically identifiable cell nucleus during the in situ hybridization.

A method of staining target chromosomal material comprising:

• (a) providing at least one labeled nucleic acid probe having a complexity greater than about 50 kb, which labeled nucleic acid probe comprises fragments which are substantially complementary to unique nucleic acid segments within the chromosomal material for which detection is desired, and providing blocking nucleic acid that comprises fragments which are substantially complementary to repetitive segments in the labeled nucleic acid;
• and
• (b) employing said labeled nucleic acid probe, blocking nucleic acid, and chromosomal DNA in in situ hybridization so that labeled repetitive segments, if present, are substantially blocked from binding to the chromosomal DNA, while hybridization of unique segments within the labeled nucleic acid probe to the chromosomal DNA is allowed, wherein blocking of the labeled repetitive segments is sufficient to permit detection of hybridized labeled nucleic acid containing unique segments, and wherein the chromosomal DNA is present in a morphologically identifiable chromosome or cell nucleus during the in situ hybridization.

A method of staining target interphase chromosomal DNA comprising:

• (a) providing at least one labeled nucleic acid probe having a complexity greater than about 50 kb which labeled nucleic acid probe comprises fragments which are substantially complementary to unique nucleic acid segments within the chromosomal DNA for which detection is desired, wherein the nucleic acid probe is substantially free of repetitive segments which are complementary to repetitive segments in the target interphase chromosomal material;
• and
• (b) employing said labeled nucleic acid probe and chromosomal DNA in in situ hybridization so that hybridization of unique segments within the labeled nucleic acid probe to the chromosomal DNA is allowed, and hybridized labeled nucleic acid containing unique segments are detected, and wherein the interphase chromosomal DNA is present in a morphologically identifiable cell nucleus during the in situ hybridization.