Indolicidin analogs with anti-microbial activity
AssigneeCouncil of Scientific and Industrial Research
InventorsChilukuri Subbalakshmi, Eruguraia Bikshapathy, Narasimhaiah Sitaram, Ramakrishnan Nagaraj
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Abstract
The present invention relates to three single tryptophan analogs ILPWKLPLLPLRR-amide (IL4) (SEQ ID NO: 1), ILPLKLPWLPLRR-amide (IL8) (SEQ ID NO: 2) and ILPLKLPLLPWRR-amide (IL11) (SEQ ID NO: 3), of Indolicidin, a cationic tridecapeptide amide found in the granules of bovine neutrophils, the analogs having the amino acid only at the 4th, 8th or 11th position from the N-terminal, with leucine at its all other conventional positions, the analogs selectively having only anti-microbial activity and no hemolytic activity of Indolicidin, and thereby providing therapeutic options and a method thereof.
Claims10
Three single tryptophan analogs of Indolicidin having amino-acid sequence of ILPWKLPLLPLRR-amide (IL4) (SEQ ID NO:
- 1), ILPLKLPWLPLRR-amide (IL8) (SEQ ID NO:
- 2) and ILPLKLPLLPWRR-amide (L11) (SEQ ID NO:
- 3), with said amino acid at positions 4, 8 and 11 respectively from N-terminal.
A method of producing three single tryptophan analogs of Indolicidin having amino-acid sequence of ILPWKLPLLPLRR-amide (IL4) (SEQ ID NO:
- 1), ILPLKLPWLPLRR-amide (IL8) (SEQ ID NO:
- 2) and ILPLKLPLLPWRR-amide (SEQ IL11) (ID NO:
- 3), with said amino acid at positions 4, 8 and 11 respectively from N-terminal, said method comprising:
- (i) synthesizing peptide sequence of said analogs with C-terminal amide, and
- (ii) purifying synthesized sequence using High Performance Liquid Chromatography HPLC.
An antibacterial method comprising contacting bacteria with one or more single tryptophan analogs of Indolicidin having an amino-acid sequence ILPWKLPLLPLRR-amide (IL4) (SEQ ID NO:
- 1), ILPLKLPWLPLRR-amide (IL8) (SEQ ID NO:
- 2) and ILPLKLPLLPWRR-amide (IL11) (SEQ ID NO:
- 3), with said amino acid at positions 4, 8 and 11 respectively from N-terminal.
Dependencies are inferred from the claim text.