Recombinant type II restriction endonucleases, MmeI and related endonucleases and methods for producing the same
AssigneeNew England Biolabs, Inc.
InventorsRichard D. Morgan, Tanya Bhatia, Theodore Davis, Lindsay Lovasco
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Abstract
In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme. This one polypeptide possesses two related enzymatic functions; namely an endonuclease activity which recognizes the DNA sequence 5′-TCC(Pu)AC-3′ and cleaves as indicated by the arrows: 5′-TCCRAC(N20)↓-3′ 3′-AGGYTG(N18)↑-5′ and a second enzymatic activity that recognizes the same DNA sequence, 5′-TCC(Pu)AC-3′, but modifies this sequence by the addition of a methyl group to prevent cleavage by the MmeI endonuclease activity.
Claims6
Isolated DNA coding for the MmeI restriction enzyme, wherein the isolated DNA is obtainable from Methylophilus methylotrophus.
A recombinant DNA vector comprising a vector into which a DNA segment coding for the MmeI restriction enzyme has been inserted.
Isolated DNA coding for the MmeI endonuclease and methyltransferase, wherein the isolated DNA is obtainable from ATCC Accession No. PTA-4521.
Dependencies are inferred from the claim text.