Polynucleotides encoding a novel testis-specific tubulin tyrosine-ligase-like protein, BGS42
AssigneeBristol-Myers Squibb Company
InventorsJohn N. Feder, Shujian Wu, Thomas C. Nelson
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Abstract
The present invention provides novel polynucleotides encoding BGS-42 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel BGS-42 polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.
Claims35
An isolated nucleic acid molecule comprising a polynucleotide sequence selected from the group consisting of:
- (a) an isolated polynucleotide encoding a polypeptide comprising amino acids 1 to 541 of SEQ ID NO:2;
- and
- (b) an isolated polynucleotide encoding a polypeptide comprising amino acids 2 to 541 of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a polynucleotide encoding a polypeptide that is at least 95.0% identical to amino acids 2 to 541 of SEQ ID NO:2, wherein percent identity is calculated using a CLUSTALW global sequence alignment using default parameters wherein said polynucleotide encodes a polypeptide that has tubulin tyrosine ligase activity.
An isolated polynucleotide encoding a polypeptide consisting of at least 50 contiguous amino acids of SEQ ID NO:2.
An isolated nucleic acid molecule comprising the cDNA clone contained in plasmid BGS-42 clone A in ATCC Deposit No. PTA-4454.
An isolated nucleic acid molecule comprising the cDNA clone contained in plasmid BGS-42 clone B in ATCC Deposit No. PTA-4454.
An isolated nucleic acid molecule comprising the cDNA clone contained in plasmid BGS-42 clone C in ATCC Deposit No. PTA-4454.
An isolated polynucleotide encoding a polypeptide comprising amino acids 73 to 365 of SEQ ID NO:2.
An isolated polynucleotide encoding a polypeptide comprising amino acids 133 to 374 of SEQ ID NO:2.
An isolated nucleic acid molecule comprising nucleotides 1 to 2057 of SEQ ID NO:27.
An isolated nucleic acid molecule comprising the nucleotide sequence provided as SEQ ID NO:9.
An isolated nucleic acid molecule comprising the nucleotide sequence provided as SEQ ID NO:10.
An isolated nucleic acid molecule comprising the nucleotide sequence provided as SEQ ID NO:11.
An isolated nucleic acid comprising a polynucleotide encoding amino acids 2 to 541 of SEQ ID NO:2, wherein the amino acid located at amino acid position 515 is a glutamic acid.
An isolated nucleic acid comprising a polynucleotide encoding amino acids 2 to 541 of SEQ ID NO:2, wherein the amino acid located at amino acid position 524 is a serine.
An isolated polynucleotide encoding a polypeptide comprising at least 394 contiguous amino acids of SEQ ID NO:2.
An isolated polynucleotide that hybridizes under stringent conditions to the polynucleotide encoding amino acids 2 to 541 of SEQ ID NO:2, wherein said stringent conditions are as follows:
- an overnight incubation at 42 degree C. in a solution comprising 50% formamide, 5×SSC (750 mM NaCl, 75 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA, followed by washing the filters in 0.1×SSC at about 65 degree C. wherein said polynucleotide encodes a polypeptide that has tubulin tyrosine ligase activity.
Dependencies are inferred from the claim text.