Production of recombinant AAV using adenovirus comprising AAV rep/cap genes
AssigneeGenovo, Inc.
InventorsHaifeng Chen, Gary Kurtzman
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Abstract
This invention relates to novel adenoviruses useful in the production of high titers of recombinant adeno-associated virus (rAAV) comprising a foreign DNA insert and methods of making these adenoviruses. The adenovirus comprises the AAV rep gene in which the p5 promoter is replaced by a minimal promoter or by no promoter. The invention also provides methods of producing high levels of rAAV as a substantially homogenous preparation and composition of rAAV.
Claims19
An adenovirus vector for the manufacture of rAAV, wherein the adenovirus vector comprises an AAV rep gene, and wherein the AAV p5 promoter is deleted upstream of the AAV rep gene and said vector contains a minimal promoter in place of the p5 promoter, wherein the minimal promoter contains a TATA box as its only regulatory element.
An adenovirus vector for the manufacture of rAAV, wherein the adenovirus vector comprises an AAV rep gene, and wherein the AAV p5 promoter is deleted upstream of the AAV rep gene and said vector contains a minimal Drosophila heat shock promoter in place of the p5 promoter.
An adenovirus vector for the manufacture of rAAV, wherein the adenovirus vector comprises an AAV rep gene, and wherein the AAV p5 promoter is deleted upstream of the AAV rep gene and said vector contains a minimal adenoviral E1b promoter in place of the p5 promoter.
Dependencies are inferred from the claim text.