Device and method for carrying out immunological marking techniques for thin-sectioned tissue
AssigneeLeica Mikrosysteme GmbH
InventorGeorge Posthuma
Life of this patent
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Abstract
The invention relates to a device and a method for carrying out in an optimized and automated manner immunological marking techniques for thin-sectioned tissue (2b). A support plate (1) which can be automatically moved by means of a computer-controlled conveying device (20) and on which several thin sections of tissue (2b) are placed on small metal screens (2) is immersed like a die into a liquid that is composed of a washing or marking solution (6) and is placed in several recesses (5) within an object support (4). The object support can also be automatically moved.
Claims33
A device for treating thin-sectioned tissues with at least one treatment liquid, said device comprising a support plate for said tissues and an object support comprising recesses for said at least one treatment liquid, said support plate disposed opposite said object support when in a treatment position and comprises an upper side and an underside with marked positions and metal screens for mounting said thin-sectioned tissues, said upper side having a plurality of magnets disposed in holes, said holes being positioned opposite said marked positions, wherein said device is adapted so that a plurality of said object supports can be automatically placed into said treatment position.
A method for treating thin-sectioned tissues on a support plate with at least one treatment liquid by the steps which comprise:
- (i) filling at least one object support comprising recesses with said at least one treatment liquid;
- (ii) mounting said thin-sectioned tissues on metal screens resting on marked and raised positions on the underside of said support plate maintained in place by magnetic means disposed in holes on the upper side of said support plate opposite said marked positions;
- (iii) bringing the object support into a treatment position;
- (iv) lowering the support plate onto the object support for contact between the treatment liquid and the thin-sectioned tissues, and
- (v) at least automatically moving the object support to a next treatment position.
Dependencies are inferred from the claim text.