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US 7,115,385 B2Granted
Media and methods for cultivation of microorganisms
AssigneeNorth Carolina State University
InventorsEdward B. Breitschwerdt, Sushama Sontakke
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Abstract
The present invention provides culture media and methods for culturing organisms, preferably microorganisms, more preferably fastidious microorganisms. Also provided are methods of isolating and detecting organisms using the inventive culture media.
Claims47
A culture medium for growing a fastidious microorganism wherein the culture medium comprises:
- free amino acids including glutamine at a concentration of 300 to 6000 mg/l;
- organic acid as a carbon source;
- hemin;
- yeast extract;
- nucleotides;
- vitamins;
- acyl homoserine lactone and
- sodium bicarbonate;
- and further wherein the culture medium is protein free.
6 more elements
Dependencies are inferred from the claim text.