Assays for amphetamine and methamphetamine
AssigneeDade Behring Inc.
InventorsYi Feng Zheng, Richard F. Parrish, Johnny Valdez, Hshiou-ting Liu
Life of this patent
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Abstract
Methods, compositions and kits are disclosed. The compounds disclosed comprise an amphetamine moiety and a methamphetamine moiety linked together by a first linking group. A second linking group depends from the first linking group and comprises a functional group. The distance of the amphetamine moiety and the methamphetamine moiety from the point of linkage of the second linking group to the first linking group is approximately the same. The compounds may be linked to labels and used in assays for the detection of amphetamine and/or methamphetamine in samples suspected of containing these drugs.
Claims44
A compound comprising an amphetamine moiety and a methamphetamine moiety linked together by a first linking group wherein a second linking group is linked to said first linking group and the distance of the amphetamine moiety and the methamphetamine moiety from the point of linkage of said second linking group to said first linking group is approximately the same and wherein said second linking group terminates in a functional group.
A compound of the formula:
- wherein:
- A is an amphetamine moiety,
- M is a methamphetamine moiety,
- L is a linking group,
- Y is a bond or a linking group and is bonded to L at a point equidistant between A and M, and
- Z is a poly(amino acid), a non-poly(amino acid) label moiety or a functional group;
- t is 1 when Z is a functional group or a non-poly(amino acid) label or, when Z is a poly(amino acid), t is an integer between 1 and the molecular weight of a poly(amino acid) divided by about 500;
- and salts thereof.
A compound of the formula:
- wherein:
- R1 is hydrogen, lower alkyl or a protecting group,
- R2 is hydrogen, lower alkyl or a protecting group,
- L1 is a linking group,
- Y1 is a bond, a functional group or a linking group and is bonded to L1 at a point equidistant between the point of attachment to each of the phenyl groups,
- Z1 is a poly(amino acid), a non-poly(amino acid) label or a functional group;
- and
- t′ is 1 when Z1 is a functional group or a non-poly(amino acid) label or, when Z1 is a poly(amino acid), t′ is an integer between 1 and the molecular weight of a poly(amino acid) divided by about 500;
- and salts thereof.
A compound of the formula:
- wherein:
- R1 and R2 are independently H or a protecting group,
- X and X′ are independently O, S, or a bond;
- D and D′ are independently alkylene or substituted alkylene;
- V and V′ are independently O, S, or a bond;
- W is CH;
- Y is NR3 wherein R3 is H or lower alkyl, O, S, or a bond;
- T is alkylene, —(C═O)alkylene, ethereal alkylene, acetamide or a bond;
- Y′ is NR3 wherein R3 is H or lower alkyl, O, S, or a bond;
- T′ is alkylene, —(C═O)alkylene, ethereal alkylene, acetamide or a bond;
- and
- Z′ is a poly(amino acid), a non-poly(amino acid) label moiety, H, Br, Cl, F, I, NH2, acetamide, or haloacetamide;
- t″ is 1 when Z′ is a functional group or a non-poly(amino acid) label or, when Z′ is a poly(amino acid), t″ is an integer between 1 and the molecular weight of a poly(amino acid) divided by about 500;
- with the proviso that X and X′ have approximately the same length, D and D′ have approximately the same length, and V and V′ have approximately the same length;
- and salts thereof.
A compound of the formula:
- wherein:
- R1′ and R2′ are independently H or a protecting group,
- X1′ and X1″ are S or O;
- Z″ is an enzyme;
- H, Br, Cl, F, I, NH2, acetamide, or haloacetamide;
- t′″ is 1 when Z″ is other than an enzyme label and, when Z″ is an enzyme label, t′″ is an integer between 1 and the molecular weight of said enzyme label divided by about 500;
- and
- n, m, p, q are each independently 1 to 5 and r and s are each independently 0 to 5;
- and salts thereof.
A compound of the formula:
- wherein:
- Z″ is an enzyme label;
- and
- t′″ is an integer between 1 and the molecular weight of said enzyme label divided by about 500.
A method far determining amphetamine and/or methamphetamine in a sample suspected of containing amphetamine and/or methamphetamine, said method comprising:
- (a) providing in combination in a medium:
- (i) said sample,
- (ii) an antibody for amphetamine,
- (iii) an antibody for methamphetamine,
- (iv) a compound of the formula:
- wherein:
- R1 and R2 are H,
- X and X′ are independently O, S, or a bond;
- D and D′ are independently alkylene or substituted alkylene;
- V and V′ are independently O, S, or a bond;
- W is CH;
- Y is O, S, a bond, or NR3 wherein R3 is H or lower alkyl;
- T is alkylene, —(C═O)alkylene, ethereal alkylene, acetamide or a bond;
- Y′ is O, S, a bond, or NR3 wherein R3 is H or lower alkyl;
- Y′ is alkylene, —(C═O)alkylene, ethereal alkylene, acetamide or a bond;
- and
- Z′ is an enzyme;
- t″ is an integer between 1 and the molecular weight of said enzyme divided by about 500;
- with the proviso that X and X′ have approximately the same length, D and D′ have approximately the same length, and V and V′ have approximately the same length;
- and
- (b) examining for the presence or amount of signal from said enzyme, the presence or amount thereof indicating the presence or amount of said amphetamine and/or methamphetamine in said sample.
A method for determining amphetamine and/or methamphetamine in a sample suspected of containing amphetamine and/or methamphetamine, said method comprising:
- (a) providing in combination in a medium:
- (i) said sample,
- (ii) an antibody for amphetamine,
- (iii) an antibody for methamphetamine,
- (iv) a compound of the formula:
- wherein:
- R1′ and R2′ are H,
- X1′ and X1″ are S or O;
- Z″ is an enzyme;
- t′″ is an integer between 1 and the molecular weight of said enzyme divided by about 500;
- and
- n, m, p, q, r and s are each independently 1 to 5;
- and
- (b) examining for the presence or amount of signal from said enzyme, the presence or amount thereof indicating the presence or amount of said amphetamine and/or methamphetamine in said sample.
A kit comprising in packaged combination:
- (i) an antibody for amphetamine,
- (ii) an antibody for methamphetamine,
- (iii) a compound of the formula:
- wherein:
- A is an amphetamine moiety,
- M is a methamphetamine moiety,
- L is a linking group,
- Y is a bond or a linking group and is bonded to L at a point equidistant between A and M,
- Z is an enzyme,
- t is an integer between 1 and the molecular weight of said enzyme divided by about 500.
Dependencies are inferred from the claim text.