Peptide biomarker and method of identification
AssigneePharmacia Corporation
InventorsDean J. Welsch, Kevin L. Duffin, Olga V. Nemirovskiy, Dawn R. Dufield, Teresa Sunyer, Carol Pearcy Howard
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Abstract
A method for identifying and quantifying peptides resulting from enzyme cleavage of collagen type II by mass spectrometric analysis to detect characteristic peptide fragments of a carboxy-terminus having known mass to charge ratios to identify the peptide having that carboxy-terminus. Identification and quantification of the peptides in a biological sample is used to assess activity of proteolytic enzymes in diseases or physiological conditions such as osteoarthritis and rheumatoid arthritis and to assess the efficacy of enzyme blocking agents.
Claims17
A method of detecting enzymatic proteolysis of a collagen, the method comprising identifying and quantifying a specific peptide proteolysis product of the collagen occurring in a biological sample or biological extract, wherein the specific peptide proteolysis product is selected from the group consisting of SEQ ID NO:
- 2, 15, 16 and 17.
A method of detecting enzymatic proteolysis of a collagen, the method comprising identifying and quantifying a specific peptide proteolysis product of the collagen occurring in a biological sample or biological extract, wherein the biological specimen or biological extract is from a human subject, the method comprising the identification and quantification of the peptide SEQ ID NO:
- 2.
A method of detecting enzymatic proteolysis of a collagen, the method comprising identifying and quantifying a specific peptide proteolysis product of the collagen occurring in a biological sample or biological extract, wherein the biological specimen or biological extract is from a human subject, the method comprising the identification and quantification of the peptide SEQ ID NO:
- 15.
A method of detecting enzymatic proteolysis of a collagen, the method comprising identifying and quantifying a specific peptide proteolysis product of the collagen occurring in a biological sample or biological extract, wherein the biological specimen or biological extract is from a human subject, the method comprising the identification and quantification of the peptide SEQ ID NO:
- 16.
A method of detecting enzymatic proteolysis of a collagen, the method comprising identifying and quantifying a specific peptide proteolysis product of the collagen occurring in a biological sample or biological extract, wherein the biological specimen or biological extract is from a human subject, the method comprising the identification and quantification of the peptide SEQ ID NO:
- 17.
A method of detecting enzymatic proteolysis of a collagen, the method comprising specifically detecting the peptide SEQ ID NO:
- 16 in a biological sample or biological extract.
A method of detecting enzymatic proteolysis of a collagen, the method comprising specifically detecting the peptide SEQ ID NO:
- 16 in a biological sample or biological extract.
A method of detecting enzymatic proteolysis of a collagen, the method comprising specifically detecting the peptide SEQ ID NO:
- 2 in a biological sample or biological extract.
A method of detecting enzymatic proteolysis of a collagen, the method comprising specifically detecting the peptide SEQ ID NO:
- 15 in a biological sample or biological extract.
A method of detecting enzymatic proteolysis of a collagen, the method comprising specifically detecting the peptide SEQ ID NO:
- 17 in a biological sample or biological extract.
Dependencies are inferred from the claim text.