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US 7,115,374 B2Granted
Compositions and methods for detecting West Nile virus
AssigneeGen-Probe Incorporated
InventorJeffrey M. Linnen
Life of this patent
Estimating the term…
Abstract
Compositions, methods and kits for detecting flavivirus nucleic acids. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers for detecting very low levels of West Nile virus nucleic acids.
Claims8
A kit for amplifying a target nucleic acid sequence that may be present in a biological sample, comprising:
- a first primer that consists of a 3′ terminal target-complementary sequence and optionally a first primer upstream sequence that is not complementary to said target nucleic acid sequence that is to be amplified, said 3′ terminal target-complementary sequence of said first primer consisting of 22–24 contiguous bases contained within SEQ ID NO:74, and said 3′ terminal target-complementary sequence of said first primer being fully contained within the sequence of SEQ ID NO:74, allowing for the presence of RNA and DNA equivalents;
- and
- a second primer that consists of a 3′ terminal target-complementary sequence and optionally a second primer upstream sequence that is not complementary to said target nucleic acid sequence that is to be amplifed, said 3′ terminal target-complementary sequence of said second primer consisting of 18–31 contiguous bases contained within SEQ ID NO:59, and said 3′ terminal target-complementary sequence of said second primer being fully contained within the sequence of SEQ ID NO:59, allowing for the presence of RNA and DNA equivalents.
Dependencies are inferred from the claim text.