US 7,115,363 B1 · Retrovirus capable of causing AIDS, means and methods for detecting it in vitro

Abstract

The invention relates to a new class of retroviruses, designated by HIV-2, of which samples have been deposited to the ECACC under numbers 87.01.1001 and 87.01.1002 and to the NCIB under numbers 12.398 and 12.399. It relates also to antigens capable to be obtained from this virus, particularly proteins p12, p16, p26 and gp140. These various antigens can be used for the diagnosis of the disease, especially by contacting these antigens with a serum of a patient submitted to the diagnosis. It relates to immunogenic compositions containing more particularly the glycoprotein gp140. Finally it concerns nucleotidic sequences, which can be used especially as hybridization probes, derived from the RNA of HIV-2.

Claims11

Dependent claims

A method of detecting HIV-2 retrovirus nucleic acid in a biological sample, said method comprising:

a) contacting said sample with an HIV-2 specific probe under stringent hybridization conditions, wherein said probe comprises an HIV-2 nucleic acid molecule, which hybridizes to HIV-2ROD genomic DNA deposited as CNCM I-352 under stringent hybridization conditions;
wherein the probe comprises an HIV-2 nucleic acid molecule obtained from nucleotides 1–380 of the U3/R region of HIV-2, nucleotides 1–1566 of the gag gene of HIV-2, nucleotides 1114–1524 of the gag gene, nucleotides 1–405 of the gag gene, nucleotides 406–1155 of the gag gene, or nucleotides 1–2673 of the env gene of HIV-2 or a fragment of said nucleic acid molecules;
b) washing the resulting hybrid;
and
c) detecting said hybrid.

2 more elements

A method of producing an HIV-2 specific hybridization probe for HIV-2 retrovirus nucleic acid, said method comprising:

a) providing a nucleic acid insert, which hybridizes to HIV-2ROD genomic DNA deposited as CNCM I-352 under stringent hybridization conditions,
wherein the insert comprises an HIV-2 nucleic acid molecule obtained from nucleotides 1–380 of the U3/R region of HIV-2, nucleotides 1–1566 of the gag gene of HIV-2, nucleotides 1114–1524 of the gag gene, nucleotides 1–405 of the gag gene, nucleotides 406–1155 of the gag gene, or nucleotides 1–2673 of the env gene of HIV-2 or a fragment of said nucleic acid molecules;
b) introducing the insert into a vector;
c) introducing said vector into a competent cellular host;
d) culturing the cellular host;
and
e) recovering the DNA recombinants.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

08/470,489Filed 6 Jun 1995 · division

Priority

FR 86 0091022 Jan 1986

Granted

3 Oct 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification17 codes

C07K 7/06
Linear peptides containing only normal peptide links: having 5 to 11 amino acids
C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
C12N 7/00
Viruses
C12Q 1/703
For retroviruses: viruses associated with AIDS

Family

Application06/835,228Parent of this continuation-in-part · filed 3 Mar 1986

Application06/916,080Parent of this continuation-in-part · filed 6 Oct 1986

Application06/933,184Parent of this continuation-in-part · filed 21 Nov 1986

Application07/003,764Parent of this continuation-in-part · filed 16 Jan 1987

Application07/150,645Parent of this continuation · filed 20 Nov 1987

US 4,839,288 AParent of this continuation-in-part · filed 13 Jun 1989

Application07/462,908Parent of this division · filed 10 Jan 1990

Application07/792,524Parent of this continuation · filed 18 Nov 1991

Application08/075,020Parent of this continuation · filed 11 Jun 1993

Application08/392,613Parent of this division · filed 22 Feb 1995

US 6,429,306 B1Parent of this division

Application08/392,613Parent of this continuation

US 5,066,782 AParent of this division

Application07/792,524Parent of this division

Application07/462,908Parent of this continuation

US 5,051,496 AParent of this continuation-in-part

Application07/150,645Parent of this continuation-in-part

US 7,115,363 B1This patent

From the US filing history. Foreign family members aren’t listed.

Retrovirus capable of causing AIDS, means and methods for detecting it in vitro

Life of this patent

Retrovirus capable of causing AIDS, means and methods for detecting it in vitro

Life of this patent

Abstract

Claims11