A method of preparing corn fiber oil from corn aleurone cells, said method comprising grinding dried corn fiber to produce ground corn fiber having a particle size of less than about 100 microns, combining said ground corn fiber with an aqueous solution having a specific gravity effective to float aleurone cells, removing the floating aleurone fraction, drying said aleurone fraction to form a dried aleurone fraction, grinding said dried aleurone fraction to form a ground aleurone fraction, extracting corn fiber oil from said ground aleurone fraction by means extraction medium selected from the group consisting of an organic solvent and supercritical fluid extraction, and separating the extracted corn fiber oil from said extraction medium.