Protein D—an IgD-binding protein of Haemophilus influenzae
OwnerArne Forsgreninventor
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Abstract
A novel surface exposed protein of Haemophilus influenzae or related Haemophilus species is described. The protein named protein D is an Ig receptor for human IgD and has an apparent molecular weight of 42,000. Protein D can be detected in all of 116 encapsulated and non-encapsulated isolates of H. influenzae studied. The protein from all strains shows in addition to the same apparent molecular weight immunogenic similarities since protein D from all strains interacts with three different mouse monoclonal antibodies and monoclonal human IgD. A method for purification of protein D is described. Cloning of the protein D gene from H. influenzae in E. coli is described as well as the nucleotide sequence and the deduced amino acid sequence.
Claims15
A purified and isolated protein D, wherein said protein D:
- is isolatable from a Haemophilus species;
- has an apparent molecular weight of 42,000 Daltons, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis under reducing conditions and comparison to molecular weight standards;
- and
- binds at least one monoclonal antibody that also binds protein D consisting of the amino acid sequence of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.