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US 7,114,287 B2Granted
Method for obtaining solid culture of Zang Zhi (Antodia camphorate)
AssigneesMing-Huang Lan, Li-Yu Wu
InventorsMing-Huang Lan, Chang-Chou Lan, Chung-Liang Lan, Chung-Min Lan
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Abstract
An incubation method for obtaining solid culture of Zang Zhi (Antrodia camphorata) including the steps of: incubating a stock strain of Zang Zhi in a PP bag for growth of mycelia, followed by an incubation in the air for fruiting the Zang Zhi body.
Claims20
A method for culturing Antrodia camphorata comprising:
- (a) culturing spawn of a wild-type fungus of Antrodia camphorata in a bag containing a medium comprising 10–70 wt % of a celluloytic substance, 10–30 wt % of a starch, 5–15 wt % of a millet, 1–10 wt % of a saccharide, 0.5–2 wt % of a phosphate and 0.1–1 wt % of a sulfite salt at a relative humidity of 60–80 wt %, the medium having a neutral pH, the culturing being carried out at a temperature of 5–32° C. in an atmosphere comprising 0.1–1 vol % carbon dioxide for a period sufficient to grow mycelium from the spawn;
- (b) removing the medium from the bag;
- (c) exposing the removed medium to air and maintaining the removed medium in the air for a period of days sufficient to form a solid fruiting body, said removed medium being maintained in the air at a temperature which differs between days and nights with the removed medium being maintained during the days at a temperature between 20° and 30° C. and during the nights at a temperature of 10–12° C., wherein the humidity of the air is between 90–95% and the air contains less than 1 vol % of carbon dioxide;
- and
- (d) recovering the solid fruiting body.
2 more elements
Dependencies are inferred from the claim text.