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US 7,112,344 B2Granted
Vapor fraction from seeds of Glycine max (L.)Merr. and composition thereof
OwnerI-Hung Chuinventor
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Abstract
The present invention mainly relates to a vapor fraction from seeds of Glycine max (L.) Merr. prepared by vaporizing a crude extract, and pharmaceutical composition thereof The present invention also provides the use of the vapor fraction in treating skin injuries, dermatological disorders, stimulating cell regeneration, and stimulating hair growth.
Claims13
A vapor fraction from seeds of Glycine max (L.) Merr., which vapor fraction is prepared by the process comprising the steps of:
- (a) providing a crude extract of the seeds in an alcohol solution containing alcohol at the concentration lower than about 15% wt. or in water;
- and
- (b) vaporizing the crude extract at a barometric pressure lower than about 1 atm and at a temperature lower than about 110° C. to obtain the vapor fraction;
- wherein a spectrogram of the 50 mL of the vapor fraction taken at 200 nm through a high performance liquid chromatography (HPLC) using Waters Sphersorb™-ODS2 column with an inner diameter (I.D.) of 4.6 mm, a length (L) of 250mm and a particle size of 5 mm comprises peaks at a retention time of 2.910, 5.190, 13.190, and 50.815 minutes with the coefficient of variation of 8%, where mobile phase is at 0 to 5 minutes is 95% H2O/5% CH3CN;
- at 5 to 30 minutes is 95% H2O/5% CH3CN in gradient, at 30 to 80 minutes is 75% H2O/25% CH3CN in gradient;
- at 80 to 100 minutes is 0% H2O/100% CH3CN in gradient;
- at 100 to 101 minutes is 0% H2O/100% CH3CN in gradient;
- and at 101 minute is 95% H2O/5% CH3CN and a flow rate is 1 mL/min.
5 more elements
Dependencies are inferred from the claim text.