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US 7,105,328 B2Granted
Methods for screening for compounds that modulate pd-1 signaling
AssigneesDana-Farber Cancer Institute, Inc., Wyeth
InventorsClive Wood, Gordon J. Freeman, Divya Chaudhary
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Abstract
Disclosed are screening assays for identifying compounds which modulate the activity of, or signaling via, B7-4, or PD-1, especially which modulate the binding of B7-4 or PD-1 to a target molecule Further disclosed are a vaccine and also other compositions which contain agents that modulate signaling via PD-1.
Claims6
A method for screening for compounds which modulate PD-1 signaling comprising:
- a) contacting a T cell activated in the presence of PD-L1 with a test compound;
- b) contacting a T cell activated in the absence of PD-L1 with the test compound;
- and
- c) determining the ability of the test compound to modulate phosphorylation of ERK 1 or ERK 2;
- wherein modulation of phosphorylation of ERK 1 or ERK 2 in the presence of PD-L1 and not in the absence of PD-L1 indicates modulation of PD-1 signaling, thereby identifying a compound which modulates PD-1 signaling.
2 more elements
A method for screening for compounds which modulate PD-1 signaling comprising:
- a) contacting a T cell activated in the presence of PD-L1 with a test compound;
- b) contacting a T cell activated in the absence of PD-L1 with the test compound;
- and
- c) determining the ability of the test compound to modulate phosphorylation of PKC-θ;
- wherein modulation of phosphorylation of PKC-θ in the presence of PD-L1 and not in the absence of PD-L1 indicates modulation of PD-1 signaling, thereby identifying a compound which modulates PD-1 signaling.
2 more elements
Dependencies are inferred from the claim text.