Self-processing plants and plant parts

A method to prepare ethanol comprising the steps of:

• a) treating a transgenic monocot plant part comprising an alpha-amylase and at least one enzyme selected from the group consisting of a glucoamylase, α-glucosidase, glucose isomerase, pullulanase, and a combination thereof, with heat for an amount of time and under conditions to activate the alpha-amylase and at least one enzyme thereby digesting polysaccharide to form oligosaccharide or fermentable sugar, wherein the plant part is obtained from a transformed plant, the genome of which is augmented with an expression cassette comprising a seed specific promotor operable linked to a polynucleotide encoding the alpha-amylase and an endoplasmic reticulum (ER) targeting sequence;
• and
• b) incubating the oligosaccharide or fermentable sugar under conditions that promote the conversion of the fermentable sugar into ethanol.

A method to prepare ethanol comprising the steps of:

• a) treating a transgenic monocot plant part comprising an alpha-amylase and at least one enzyme selected from the group consisting of glucoamylase, α-glucosidase, glucose isomerase, pullulanase, and a combination thereof, under conditions to activate alpha-amylase and the at least one enzyme thereby digesting polysaccharide to form fermentable sugar, wherein the plant part is obtained from a transformed plant, the genome of which is augmented with an expression cassette comprising a seed specific promoter operably linked to a polynucleotide encoding the alpha-amylase and an endoplasmic reticulum (ER) targeting sequence;
• and
• b) incubating the fermentable sugar under conditions that promote the conversion of the fermentable sugar into ethanol.