Deposition of samples and sample matrix for enhancing the sensitivity of matrix assisted laser desorption/ionization mass spectrometry
AssigneeWisconsin Alumni Research Foundation
InventorsDavid M. Barnes, Bradley J. Larson, Max G. Lagally, Martha M. Vestling
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Abstract
The present invention relates to small sample spots for MALDI targets and methods for producing the same. The sample spots are composed of a layer of matrix material and a layer of analyte. In some instances, the samples spots have diameters of no more than 50 micrometers or even smaller. The sample spots are deposited onto a MALDI target substrate using ultrasonic deposition from a nozzle.
Claims20
A method for producing a sample spot on a MALDI target substrate, the method comprising:
- (a) providing a dispenser having a nozzle which circumferentially surrounds an interior passage extending between a dispensing end and an opposite end;
- (b) introducing a first fluid into the interior passage of the nozzle, the first fluid comprising either an analyte solution or a MALDI matrix solution;
- (c) ultrasonically actuating the nozzle at a portion of its circumference at a frequency sufficient to deposit the first fluid from the dispensing end onto the substrate;
- (d) introducing a second fluid into the interior passage of the nozzle wherein the second fluid comprises either the analyte solution or the MALDI matrix solution, whichever was not introduced as the first fluid;
- and
- (e) ultrasonically actuating the nozzle at a portion of its circumference at a frequency sufficient to deposit the fluid from the dispensing end onto the first fluid on the substrate.
A MALDI target comprising multiple sample spots having diameters of no more than 50 micrometers disposed on a substrate, the target comprising spots of crystallized matrix material disposed on the substrate and a sheet comprising an analyte disposed over the spots of crystallized matrix material.
A MALDI target comprising multiple sample spots having diameters of no more than 50 micrometers disposed on a substrate, the target comprising one or more strips comprising an analyte disposed over the substrate and one or more strips of crystallized matrix material disposed over the substrate;
- wherein the sample spots are defined by overlapping portions of the strips comprising the analyte and the strips of crystallized matrix material.
A MALDI target comprising multiple sample spots having diameters of no more than 50 micrometers disposed on a substrate, the target comprising a sheet comprising an analyte disposed on the substrate and spots of crystallized matrix material disposed on the sheet comprising the analyte.
Dependencies are inferred from the claim text.