Plant retroelements and methods related thereto
AssigneeIowa State University Research Foundation, Inc.
InventorsDavid A. Wright, Daniel F. Voytas
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Abstract
The present invention provides nucleic acids, as well as vectors, cells, and plants (including plant parts, seeds, and embryos) containing the nucleic acids. In particular, molecular tools are provided in the form of nucleic acids that encode reverse transcriptases. The invention also features methods for manipulating such nucleic acids. In addition, the invention features methods to introduce nucleic acids containing retroelements or retroelement sequences into cells.
Claims7
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a reverse transcriptase, said reverse transcriptase comprising the following amino acid motifs:
- (a) Thr-Val/Ile-Val/Ile-Xaa-Xaa-Xaa-Xaa-Xaa-Asp/Glu-Leu-Val/Ile (SEQ ID NO:191) between reverse transcriptase domains 1 and 2;
- and
- (b) Phe-Met-Asp-Asp-Phe (SEQ ID NO:197) within reverse transcriptase domain 5.
Dependencies are inferred from the claim text.