Cloning and characterization of the broad-spectrum resistance gene Pi2
AssigneeThe Ohio State University
InventorsShaohong Qu, Guo-liang Wang, Bo Zhou
Life of this patent
Estimating the term…
Abstract
Compositions and methods for enhancing or creating plant disease resistance to plant pests are provided. Transforming a plant with a novel rice Pi2-like disease resistance gene of the invention enhances disease resistance of the plant. Transformed plants, plant cells, tissues, and seed having enhanced disease resistance are also provided.
Claims21
An isolated nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule comprising the sequence set forth in SEQ ID NO:7 or the full length complement thereof;
- b) a nucleic acid molecule that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 8;
- and,
- c) a nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide that confers Blast resistance to a plant, said sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:7.
A method for creating or enhancing Blast resistance in a plant, said method comprising transforming said plant with a nucleic acid molecule and expressing said nucleic acid molecule in the plant, wherein said nucleic acid molecule is selected from the group consisting of:
- a) a nucleic acid molecule comprising the sequence set forth in SEQ ID NO:7;
- b) a nucleic acid molecule that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 8;
- and,
- c) a nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide that confers Blast resistance to in the plant, said sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
- 7.
A plant stably transformed with a DNA construct comprising a nucleic acid molecule operably linked to a promoter that drives expression of a coding sequence in a plant cell, wherein said nucleic acid molecule is selected from the group consisting of:
- a) a nucleic acid molecule comprising the sequence set forth in SEQ ID NO:7 or a full length complement thereof;
- b) a nucleic acid molecule that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:8;
- and,
- c) a nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide that confers Blast resistance to a plant, said sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:7.
Dependencies are inferred from the claim text.