Reduction of carbonyl compounds using the carbonyl reductase of Kluyveromyces marxianus
AssigneeKorea Institute of Science and Technology
InventorsHun Yeong Koh, Kyung Il Choi, Yong Seo Cho, Ae Nim Pae, Joo Hwan Cha, Ye Sun Han, Jong Soo Lee, Hong Chul Yun
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Abstract
A compound represented by a general formula (Ia) or (Ib) and a stereo-selective preparation method thereof using a carbonyl reductase which is separated from Kluyveromyces marxianus. The compound can be prepared by reduction of substituted β-keto ester and can be used as an intermediate in preparing β-lactam group antibiotics.
Claims13
A compound represented by the following formula (Ia) or (Ib):
- wherein, R represents a saturated or unsaturated alkyl group selected from the group consisting of methyl, ethyl, propyl, isopropyl, isobutyl and allyl, or an aryl group;
- and
- R1, R2, R3 and R4 are independently selected from the group consisting of hydrogen atom, a halogen atom, an alkyl group having 1–4 carbon atoms, hydroxy, an alkoxy group having 1–4 carbon atoms, an ester group, phenyl and combinations thereof wherein at least one of R1, R2, R3 and R4 is not hydrogen or methyl.
A preparation method of the compound represented by the formula (Ia) or (Ib) by reducing the compound represented by the formula (VI) with a reductase of Kluyveromyces marxianus
- wherein, R represents a saturated or unsaturated alkyl group selected from the group consisting of methyl, ethyl, propyl, isopropyl, isobutyl and allyl, or an aryl group;
- and
- R1, R2, R3 and R4 are independently selected from the group consisting of hydrogen atom, a halogen atom, an alkyl group having 1–4 carbon atoms, hydroxy, an alkoxy group having 1–4 carbon atoms, an ester group, phenyl and combinations thereof.
Dependencies are inferred from the claim text.