Use of mixed duplex oligonucleotides to effect localized genetic changes in plants

The method of claim which further comprises making seeds from the plant or from progeny of the plant.

A method of making a localized mutation in an ALS gene in a plant cell having a cell wall comprising the steps of:

• (a) perforating the cell walls of a population of plant cells;
• (b) introducing a recombinagenic oligonucleobase, which contains a first homologous region which has a sequence identical to the sequence of at least 6 base pairs of a first fragment of the ALS gene and a second homologous region which has a sequence identical to the sequence of at least 6 base pairs of a second fragment of the ALS gene, and an intervening region which contains at least 1 nucleobase heterologous to the ALS gene, which intervening region connects the first homologous region and the second homologous region;
• (c) identifying a cell of the population of plant cells having a mutation located between the first and second fragments of the ALS gene.