Method for cell culture
AssigneeFuji Photo Film Co., Ltd.
InventorsHirohiko Tsuzuki, Kazuhiro Aikawa, Akiko Matsuura
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Abstract
A method for detaching a carrier for cell culture from cultured cells formed on a surface of said carrier, which includes the step of bringing the carrier for cell culture into contact with a compound represented by the following formula (I) or a polyphosphoric acid or a salt thereof: MO3P-L11-PO3M wherein L11 represents a divalent bridging group; and M represents a hydrogen atom or a cation. The carrier preferably includes a calcium alginate layer and a cell adhesion gel layer.
Claims10
A method for detaching a carrier for cell culture from cultured cells formed on a surface of said carrier for cell culture, which comprises the step of bringing the carrier for cell culture into contact with a compound represented by the following formula (I) or a polyphosphoric acid or a salt thereof:
- MO3P-L11-PO3M
- wherein L11 represents at least one substituted or unsubstituted divalent hydrocarbon group;
- and M represents hydrogen atom or a cation, wherein the carrier for cell culture comprises a calcium alginate gel layer, to thereby detach the carrier for cell culture from the cultured cells.
A method for culturing a layer of cells by using a carrier for cell culture which comprises the steps of:
- (1) culturing cells on a carrier for cell culture comprising a calcium alginate gel layer;
- and
- (2) bringing the cultured cells and carrier for cell culture into contact with a compound represented by the following formula (I) or a polyphosphoric acid or a salt thereof,
- MO3P-L11-PO3M
- wherein L11 represents at least one substituted or unsubstituted divalent hydrocarbon group;
- and M represents hydrogen atom or a cation, to thereby detach the carrier from the cultured layer of cells.
A method for transferring a cell culture, which comprises the steps of:
- (1) culturing cells on a first carrier for cell culture comprising a calcium alginate gel layer,
- (2) bringing the cell culture into contact with a surface of a second carrier for cell culture with weighting such that the cells adhere to both surfaces;
- and
- (3) bringing the cell culture obtained in the aforementioned step (2) into contact with a compound represented by the following formula (I) or a polyphosphoric acid or a salt thereof;
- MO3P-L11-PO3M
- Wherein L11 represents at least one substituted or unsubstituted divalent hydrocarbon group;
- and M represents hydrogen atom or a cation, to detach the first carrier for cell culture.
A method for laminating cultured cell layers, which comprises the steps of:
- (1) culturing cells on a carrier for cell culture comprising a calcium alginate gel layer to provide a first cultured cell layer;
- (2) bringing the first cultured cell layer into contact with a second cultured cell layer with weighting such that the first cell layer and the second cell layer adhere to each other;
- and
- (3) bringing the adhered cell layers and carrier for cell culture obtained in the aforementioned step (2) into contact with a compound represented by the following formula (I) or a polyphosphoric acid or a salt thereof:
- MO3P-L11-PO3M
- wherein L11 represents at least one substituted or unsubstituted divalent hydrocarbon group;
- and M represents hydrogen atom or a cation, to thereby detach the carrier for cell culture and obtain laminated cultured cell layers.
Dependencies are inferred from the claim text.