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US 7,094,595 B2Granted
Label-free high-throughput optical technique for detecting biomolecular interactions
AssigneeSRU Biosystems, Inc.
InventorsBrian Cunningham, Jane Pepper, Bo Lin, Peter Li, Homer Pien
Life of this patent
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Abstract
Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.
Claims71
A biosensor comprising:
- (a) a two-dimensional grating;
- (b) a substrate that supports the two-dimensional grating;
- wherein the refractive index of the two-dimensional grating is greater than the refractive index of the substrate;
- and
- (c) one or more specific binding substances immobilized on the surface of the two-dimensional grating opposite of the substrate layer;
- wherein the one or more specific binding substances are bound to their binding partners and wherein one or more specific binding substances and their binding partners are detection label-free wherein, when the biosensor is illuminated a resonant grating effect is produced on a reflected radiation spectrum, and wherein the depth and period of the two-dimensional grating are less than the wavelength of the resonant grating effect.
3 more elements
An array of polynucleotides comprising:
- (a) a two-dimensional grating;
- (b) a substrate that supports the two-dimensional grating;
- wherein the refractive index of the two-dimensional grating is greater than the refractive index of the substrate;
- and
- (c) one or more types of polynucleotides attached at distinct locations of the two-dimensional grating opposite the substrate;
- wherein the one or more polynucleotides are bound to one or more specific binding substances, and wherein the one or more polynucleotides and the one or more specific binding substances are detection label-free;
- wherein, when the array of polynucleotides is illuminated a resonant grating effect is produced on the reflected radiation spectrum, wherein the depth and period of the two-dimensional grating are less than the resonant grating effect wavelength.
4 more elements
An array of polynucleotides comprising:
- (a) a two-dimensional grating;
- (b) a substrate that supports the two-dimensional grating;
- wherein the refractive index of the two-dimensional grating is greater than the refractive index of the substrate;
- and
- (c) a cover layer on a surface of the two-dimensional grating opposite of the substrate;
- (d) one or more types of polynucleotides attached at distinct locations to the cover layer,
- (e) wherein the one or more types of polynucleotides are bound to their binding partners and wherein the one or more types of polynucleotides and their binding partners are detection label-free,
- wherein, when the array is illuminated a resonant grating effect is produced on the reflected radiation spectrum, wherein the depth and period of the two-dimensional grating are less than the resonant grating effect wavelength.
5 more elements
Dependencies are inferred from the claim text.