Back to results
US 7,094,579 B2Granted
Eukaryotic signal sequences for prokaryotic expression
AssigneeXoma Technology Ltd.
InventorsJeff Gray, Joe Buechler, Uday Kumar Veeramallu
Life of this patent
Estimating the term…
Abstract
The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences.
Claims12
A method of expressing a Fab fragment, comprising:
- providing a culture of bacterial cells containing a vector comprising a polynucleotide encoding an antibody heavy chain and an antibody light chain each operably linked to a eukaryotic signal sequence, wherein the eukaryotic signal sequence encodes a signal peptide sequence as shown in MRTLAILAAILLVALQAQA (SEQ ID NO:2) or MKILILGIFLFLCSTPAWA (SEQ ID NO:1),
- whereby the antibody heavy chain and light chain and their linked signal sequences are expressed, secreted to the periplasm, the signal peptide sequences are processed from the heavy and light chains, and the heavy and light chains assemble to form a Fab fragment which specifically binds to a target molecule;
- and
- recovering the Fab fragment from the culture of bacterial cells.
1 more element
A method of expressing a polypeptide in a bacterial cell, comprising
- providing a culture of bacterial cells containing a vector comprising a polynucleotide encoding the polypeptide, wherein the polynucleotide is operably linked to a eukaryotic signal sequence which encodes a signal peptide selected from the group consisting of MRTLAILAAILLVALQAQA (SEQ ID NO:2) or MKILILGIFLFLCSTPAWA (SEQ ID NO:1),
- whereby the polynucleotide and the signal sequence are expressed, secreted to the periplasm, and expressed signal peptide sequence is processed from the polypeptide expressed from the polynucleotide;
- and
- recovering the polypeptide from the culture of bacterial cells.
1 more element
Dependencies are inferred from the claim text.