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AssigneeBoard of Regents, The University of Texas System
InventorsBarrett R. Harvey, George Georgiou, Brent L. Iverson
Estimating the term…
The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating binding proteins capable of binding small molecules and peptides. In the technique, libraries of candidate binding proteins, such as antibody sequences, are expressed in the periplasm of gram negative bacteria and mixed with a labeled ligand. In clones expressing recombinant polypeptides with affinity for the ligand, the concentration of the labeled ligand bound to the binding protein is increased and allows the cells to be isolated from the rest of the library. Where fluorescent labeling of the target ligand is used, cells may be isolated by fluorescence activated cell sorting (FACS). The approach is more rapid than prior art methods and avoids problems associated with the outer surface-expression of ligand fusion proteins employed with phage display.
A method of obtaining a bacterium comprising a nucleic acid sequence encoding a binding polypeptide having specific affinity for a target ligand comprising the steps of:
A method of obtaining a bacteria comprising a nucleic acid sequence encoding at least a first binding polypeptide having specific affinity for a target ligand comprising the steps of:
A method of obtaining a nucleic acid sequence encoding a binding polypeptide having a specific affinity for a target ligand comprising the steps of:
Dependencies are inferred from the claim text.