21612, novel human dehydrogenases
AssigneeMillennium Pharmaceuticals, Inc.
InventorRachel Meyers
Life of this patent
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Abstract
The present invention relates to newly identified human ADHs belonging to the superfamily of mammalian alcohol dehydrogenases. The invention also relates to polynucleotides encoding the ADHs. The invention further relates to methods using the ADH polypeptides and polynucleotides as a target for diagnosis and treatment in ADH-mediated or -related disorders. The invention further relates to drug-screening methods using the ADH polypeptides and polynucleotides to identify agonists and antagonists for diagnosis and treatment. The invention further encompasses agonists and antagonists based on the ADH polypeptides and polynucleotides. The invention further relates to procedures for producing the ADH polypeptides and polynucleotides.
Claims26
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:8;
- b) the nucleotide sequence of the cDNA insert of the plasmid deposited with ATCC as Patent Deposit Number PTA-2170;
- c) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:7;
- d) a nucleotide sequence encoding the amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Patent Deposit Number PTA-2170;
- and
- e) a nucleotide sequence complementary to a nucleotide sequence of a), b), c), or d).
An isolated nucleic acid molecule having a nucleotide selected from the group consisting of:
- a) a nucleotide sequence encoding a polypeptide having dehydrogenase activity, wherein said nucleotide sequence has at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO:8;
- and
- b) a nucleotide sequence complementary to the nucleotide sequence of a).
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence encoding a polypeptide having dehydrogenase activity, wherein the complement of said nucleotide sequence hybridizes under stringent conditions to the nucleotide sequence set forth in SEQ ID NO:8, said stringent conditions comprising hybridization at about 45° C., followed by at least one wash in 0.2×SSC/0.1% SDS at 65° C.;
- and
- b) a nucleotide sequence complementary to the nucleotide sequence of a).
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence encoding a fragment of the amino acid sequence set forth in SEQ ID NO:7, wherein said fragment has dehydrogenase activity and consists of at least 139 contiguous amino acids of SEQ ID NO:7;
- and
- b) a nucleotide sequence encoding a fragment of the amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Patent Deposit Number PTA-2170, wherein the fragment has dehydrogenase activity and consists of at least 139 contiguous amino acids of the amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Patent Deposit Number PTA-2170;
- and
- c) a nucleotide sequence complementary to the nucleotide sequence of a) or b).
Dependencies are inferred from the claim text.