Isolation and composition of novel glycosidases
AssigneeNew England Biolabs, Inc.
InventorsSharon T. Wong-Madden, Ellen P. Guthrie, David Landry, Christopher H. Taron, Chudi Guan, Phillips W. Robbins
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Abstract
Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNacβ1-x, Galα1-3R, Galα1-6R, Galβ1-3R, Fucα-2R, Fucα1-3R, Fucα1-4R, Manα1-2R, Manα1-3R, Manα1-6R, Manβ1-4R, Xylβ1-2R, Glcβ1-4R, and Galβ1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.
Claims5
A method for cleaving a glycosidic bond in a carbohydrate, comprising the steps of:
- (a) adding to the carbohydrate, a glycosidase of defined substrate specificity obtainable from a Xanthomonas species wherein the glycosidase is selected from a β1-3>>4 galactosidase, an α1-2,3 mannosidase, a β-glucosidase, an α1-3,4 fucosidase, an α1-2 fucosidase, a β-N-acetylglucosaminidase, β-N-Acetylglucosaminidase, an α1-6 mannosidase, an α1-3,6 galactosidase, an α1-3,6 mannosidase, a β-xylosidase and a β-mannosidase;
- and
- (b) cleaving the glycosidic bond between constituent monosaccharides of the carbohydrate by means of the glycosidase.
Dependencies are inferred from the claim text.