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US 7,094,560 B2Granted
Stabilization of T4 endonuclease V
AssigneeApplied Genetics Incorporated Dermatics
InventorsAnne B. Brown, David A. Brown, Daniel B. Yarosh
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Abstract
The invention provides T4 endonuclease V compositions that exhibit enhanced stability, including stability at non-refrigerated temperatures, through reduced activity of cryptic proteases. Methods for detecting cryptic protease activity and methods for reducing such activity are also provided.
Claims10
A method for detecting protease contaminants in a T4 endonuclease V composition comprising:
- (i) preparing a test solution comprising an aqueous buffer, a sample of the T4 endonuclease V composition, and a labeled reporter peptide whose amino acid sequence is Pro Leu Ser Arg Thr Leu Ser Val Ala Ala Lys (SEQ ID NO:1);
- (ii) incubating the test solution at 25° C. for a period of at least 7 days;
- and
- (iii) determining the percentage of degraded reporter peptide after step (ii).
1 more element
Dependencies are inferred from the claim text.