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US 7,094,559 B1Granted
Assay to identify estrogen receptor dependent ligands that regulate the hepatic lipase promoter
AssigneeWyeth
InventorsDouglas C. Harnish, Marshall S. Scicchitano, Sotirios K. Karathanasis, Chu-Lai Hsiao
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Abstract
The present invention relates to an assay system and method for testing compounds for their ability to regulate the hepatic lipase (HL) promoter. In particular, the invention relates to the identification of estrogen receptor ligands having this activity. Compounds that inhibit HL promoter activity are useful as leads, or on their own, to develop therapeutics in the prevention of heart disease.
Claims30
An isolated cell comprising
- (i) a first exogenous nucleic add molecule which encodes an estrogen receptor;
- (ii) a second exogenous nucleic acid molecule which encodes a CCAAT/enhancer-binding protein (C/EBP) transcription factor;
- and
- (iii) a reporter gene operatively associated with a hepatic lipase (HL) promoter.
1 more element
Dependencies are inferred from the claim text.