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US 7,094,541 B2Granted
Assay for detection of human parvovirus B19 nucleic acid
AssigneeGen-Probe Incorporated
InventorsSteven T. Brentano, Margarita Batranina-Kaminsky, Cynthia S. Hasselkus-Light, Daniel P. Kolk
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Abstract
Nucleic acid oligomers specific for human parvovirus B19 genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus B19 nucleic acid in biological specimens is disclosed. Compositions for detecting the presence of parvovirus B19 genomic DNA in human biological specimens are disclosed.
Claims25
A combination of at least two separate nucleic acid oligomers wherein the first oligomer is selected from the group consisting of:
- a) SEQ ID NO:
- 24,
- b) the complete complement of SEQ ID NO:
- 24, and
- c) the RNA equivalent of the sequence of a) or b),
- wherein the first oligomer optionally includes a promoter sequence joined to the 5′ terminus of the sequence;
- and wherein the second oligomer is selected from the group consisting of:
- d) SEQ ID NO:
- 13,
- e) the complete complement of SEQ ID NO:
- 13, and
- f) the RNA equivalent of the sequence of d) or e).
9 more elements
A method of detecting human parvovirus B19 nucleic acid in a biological sample, comprising the steps of:
- providing a biological sample containing parvovirus B19 nucleic acid;
- amplifying in vitro a portion of the parvovirus B19 nucleic acid by using at least one nucleic acid polymnerase activity and at least one first amplification oligomer and one second amplification oligomer, selected from the group consisting of
- a first amplification oligomer consisting of SEQ ID NO:23 or SEQ ID NO:24 optionally including a promoter sequence joined to a 5′ terminus of SEQ ID NO:24, and
- a second amplification oligomer consisting of SEQ ID NO:13;
- and
- detecting an amplified product of the parvovirus B19 nucleic acid by using a labeled detection probe that hybridizes specifically with the amplified product, thereby indicating presence of parvovirus B19 nucleic acid in the biological sample.
3 more elements
Dependencies are inferred from the claim text.