Purification of substances by reaction affinity chromatography
OwnerRobert C. Corcoraninventor
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Abstract
The invention relates to the separation of at least one target from a sample composition using a naturally reversible reaction comprising the formation of at least one covalent bond. One embodiment of the invention is a chromatographic method in which the affinity of a stationary phase for at least one target is based on the equilibrium constant of such a naturally reversible reaction between the stationary phase and the target.
Claims46
A method of separating a target from a sample composition containing the target, comprising the following steps:
- (a) contacting the sample composition with a reactive affinity molecule attached to a phase separating group, the reactive affinity molecule comprising a reactive functional group, and the reactive affinity molecule reacting with the target to farm an adduct by forming a covalent bond between the target and the reactive functional group, where the reaction forming the adduct is reversible under the conditions of the contacting without the addition of a reagent acting at the covalent bond, to reform the target and reactive affinity molecule
- (b) separating the adduct from the sample composition;
- (c) contacting the adduct with an eluent to regenerate the target and the reactive affinity molecule;
- and
- (d) separating the target from the reactive affinity molecule.
A method for isolating thebaine from a sample composition, comprising reversibly separating the thebaine from the sample composition by reverse affinity chromatography using with a reactive affinity molecule of the formula:
- where Y and Z are each independently chosen from H, alkyl groups, aryl groups, heteroaryl groups, framework groups, reactivity modifier groups, and framework groups with reactivity modifier groups;
- and
- is a phase separating group.
Dependencies are inferred from the claim text.