Composition for the preservation of viruses
OwnerKerrie Setiawan, Fiona Helen Cameroninventors
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Abstract
This invention relates to a composition for the preservation of a virus, the composition including a virus, a lipid and a cryoprotectant.
Claims55
A composition for the preservation of a virus, said composition comprising a virus, a polycationic lipid that has a hydrophilic moiety that includes two or more groups derived from positively charged amino acids, and a cryoprotectant,
- wherein the polycationic lipid has the following chemical formula:
- or a salt thereof,
- wherein X is a positively-charged hydrophilic moiety;
- Y is a spacer having a carbon chain length equivalent to 1 to 30 carbon-carbon single covalent bonds or is absent;
- and R1, R2, and R3 are the same or different acyl groups of a fatty acid.
A method of producing a composition for the preservation of a virus, said method comprising preparing a liquid composition comprising a virus, a polycationic lipid that has a hydrophilic moiety that includes two or more groups derived from positively charged amino acids, and a cryoprotectant,
- wherein the polycationic lipid has the following chemical formula:
- or a salt thereof,
- wherein X is a positively-charged hydrophilic moiety;
- Y is a spacer having a carbon chain length equivalent to 1 to 30 carbon-carbon single covalent bonds or is absent;
- and R1, R2, and R3 are the same or different acyl groups of a fatty acid.
A composition for the preservation of a virus, said composition comprising a virus, a polycationic lipid that has a hydrophilic moiety that includes two or more groups derived from positively charged amino acids, and a cryoprotectant,
- wherein the virus is storage stable when the composition is stored as a frozen liquid, and
- wherein the polycationic lipid has the following chemical formula:
- or a salt thereof,
- wherein X is a positively-charged hydrophilic moiety;
- Y is a spacer having a carbon chain length equivalent to 1 to 30 carbon-carbon single covalent bonds or is absent;
- and R1, R2, and R3 are the same or different acyl groups of a fatty acid.
A composition for the preservation of a virus, said composition comprising a virus, a polycationic lipid that has a hydrophilic moiety that includes two or more groups derived from positively charged amino acids, and a cryoprotectant,
- wherein the virus is stable to freeze-thawing, and
- wherein the polycationic lipid has the following chemical formula:
- or a salt thereof,
- wherein X is a positively-charged hydrophilic moiety;
- Y is a spacer having a carbon chain length equivalent to 1 to 30 carbon-carbon single covalent bonds or is absent;
- and R1, R2, and R3 are the same or different acyl groups of a fatty acid.
Dependencies are inferred from the claim text.