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US 7,090,847 B1Granted
Mammalian cytokines; related reagents and methods
AssigneeSchering Corporation
InventorsBirgit Oppmann, Rene De Waal Malefyt, Donna M. Rennick, Robert A. Kastelein, Maria T. Wiekowski, Sergio A. Lira, Satwant K. Narula
Life of this patent
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Abstract
Purified genes encoding cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using said reagents and diagnostic kits are also provided.
Claims10
A method for preparing an antibody or a fragment thereof that comprises the antigen binding site of the antibody, wherein said antibody binds to:
- (i) a mammalian IL-B30/p40 complex comprising an IL-B30 subunit (SEQ ID NO:2 or 4) and a p40 subunit (SEQ ID NO:6 or 8), or
- (ii) a fusion protein comprising the IL-B30 subunit (SEQ ID NO:2 or 4) and the p40 subunit (SEQ ID NO:6 or 8);
- said method consists of:
- (a) raising an antibody to said IL-B30/p40 complex or a polypeptide that comprises at least 11 contiguous amino acids from said p40 subunit and at least 11 contiguous amino acids from the IL-B30 subunit,
- (b) confirming that said antibody binds to the IL-B30/p40 complex or fusion protein;
- and
- (c) optionally preparing said fragment from said antibody.
4 more elements
A method of selecting an antibody or fragment thereof that specifically binds to:
- (i) a mammalian IL-B30/p40 complex comprising an IL-B30 subunit (SEQ ID NO:2 or 4) and a p40 subunit (SEQ ID NO:6 or 8), or
- (ii) a fusion protein comprising the IL-B30 subunit (SEQ ID NO:2 or 4) and the p40 subunit (SEQ ID NO:6 or 8);
- wherein the antibody or fragment thereof does not bind the IL-B30 or p40 subunit alone, said method comprising
- (a) providing candidate antibodies or fragments thereof that bind to the IL-B30/p40 complex or fusion protein;
- and
- (b) detecting if the candidate antibodies or fragments thereof bind the IL-B30 or p40 subunit, and selecting the antibody or fragment thereof that does not bind the IL-B30 or p40 subunit.
3 more elements
Dependencies are inferred from the claim text.