Fusion protein capable of binding VEGF
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsThomas J. Daly, James P. Fandl, Nicholas J. Papadopoulos
Life of this patent
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Abstract
Nucleic acid molecules and multimeric proteins capable of binding vascular endothelial growth factor (VEGF). VEGF mini-traps are disclosed which are therapeutically useful for treating VEGF-associated conditions and diseases, and are specifically designed for local administration to specific organs, tissues, and/or cells.
Claims16
An isolated nucleic acid molecule encoding a fusion polypeptide capable of binding vascular endothelial growth factor (VEGF) consisting of components (R1R2)X, and a multimerizing component (MC) capable of interacting with another MC to form a multimeric structure, wherein X≧1, R1 is VEGF receptor component Ig domain 2 of Flt-1 consisting of amino acids 27–126 of SEQ ID NO:
- 8 or 27–129 of SEQ ID NO:
- 10, R2 is Ig domain 3 of Flk-1 consisting of amino acids 127–228 of SEQ ID NO:
- 8 or 130–231 of SEQ ID NO:
- 10, and MC is an amino acid sequence between 1 to about 200 amino acids in length having at least one cysteine residue.
A replicable expression vector comprising a nucleic acid molecule encoding a fusion protein which binds vascular endothelial growth factor (VEGF), wherein the fusion protein consists of a first receptor component, a second receptor component, and a multimerizing component, wherein the first receptor component is amino acids 27–126 of SEQ ID NO:8 or 27–129 of SEQ ID NO:10, the second receptor component is amino acids 127–228 of SEQ ID NO:8 or 130–231 of SEQ ID NO:10, and the multimerizing component is an amino acid sequence between 1 to about 200 amino acids having at least one cysteine residue.
An isolated nucleic acid molecule encoding a fusion polypeptide which binds vascular endothelial growth factor (VEGF), consisting of a first receptor component, a second receptor component, and a multimerizing component, wherein the first receptor component is amino acids 27–126 of SEQ ID NO:8 or 27–129 of SEQ ID NO:10, the second receptor component is amino acids 127–228 of SEQ ID NO:8 or 130–231 of SEQ ID NO:10, and the multimerizing component is an amino acid sequence between 1 to about 200 amino acids having at least one cysteine residue.
A vascular endothelial growth factor (VEGF) trap, comprising a dimer which binds VEGF consisting of two fusion polypeptides, each fusion polypeptide consisting of a first receptor component, a second receptor component, and a multimerizing component (MC), wherein the first receptor component is amino acids 27–126 of SEQ ID NO:8 or 27–129 of SEQ ID NO:10, the second receptor component is amino acids 127–228 of SEQ ID NO:8 or 130–231 of SEQ ID NO:10, and wherein the MC is XCXC (SEQ ID NO:3).
A vascular endothelial growth factor (VEGF) trap, comprising a dimer which binds VEGF consisting of two fusion polypeptides, each fusion polypeptide consisting of a first receptor component, a second receptor component, and a multimerizing component (MC), wherein the first receptor component is amino acids 27–126 of SEQ ID NO:8 or 27–129 of SEQ ID NO:10, the second receptor component is amino acids 127–228 of SEQ ID NO:8 or 130–231 of SEQ ID NO:10, and wherein the MC is ACGC (SEQ ID NO:4).
Dependencies are inferred from the claim text.