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US 7,087,371 B2Granted
Material and methods for the production of sperm and analysis thereof
AssigneeThe Trustees of the University of Pennsylvania
InventorsIna Dobrinski, Stefan Schlatt, Ali Honaramooz, Hans Scholer
Life of this patent
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Abstract
Provided herein are methods of producing viable sperm from neonatal mammalian testes which have been engrafted in mice.
Claims13
A method for producing sperm in a recipient animal, comprising:
- a) obtaining a graft of testes tissue from a donor animal;
- b) maintaining said graft in a suitable culture medium until transplant;
- c) implanting said graft ectopically under the skin of an immunocompromised animal;
- d) maintaining said graft in recipient animal so that spermatogenesis may occur so as to produce sperm within graft;
- e) harvesting said sperm from said graft;
- wherein said donor animal and said recipient animal are different species.
3 more elements
A method for identifying the effect of an agent on germ cell differentiation and/or spermatogenesis, comprising:
- a) obtaining a graft of testis tissue from a donor animal;
- b) maintaining said graft in a suitable culture medium until transplant;
- c) implanting said graft ectopically under the skin of a pair of immunocompromised recipient animals;
- d) administering an agent suspected of affecting germ cell differentiation and/or spermatogenesis to one of said pair of recipient animals;
- e) maintaining said grafts in said recipient animals so that germ cell differentiation and/or spermatogenesis may occur;
- f) harvesting the grafts from each of said recipient animals;
- and
- g) determining the difference in germ cell differentiation and/or spermatogenesis in the graft obtained from the treated recipient animal compared to the graft obtained from the untreated recipient animal to identify effects of said agent on germ cell differentiation and/or spermatogenesis;
- wherein said donor animal is of a different species than the recipient animals.
6 more elements
A method for identifying the effect of an agent on germ cell differentiation and/or spermatogenesis, comprising:
- a) administering an agent suspected of affecting germ cell differentiation and/or spermatogenesis to one of a pair of donor animals;
- b) obtaining a graft of testis tissue from each of said donor animals;
- c) maintaining said grafts in a suitable culture medium until transplant;
- d) implanting said grafts ectopically under the skin of an immunocompromised recipient animal;
- e) maintaining said grafts in said recipient animal so that germ cell differentiation and/or spermatogenesis may occur;
- f) harvesting the grafts from said recipient animal;
- and
- g) determining the difference in germ cell differentiation and/or spermatogenesis in the graft derived from treated the treated donor animal compared to the graft derived from the untreated donor animal to identify the effect of said agent on germ cell differentiation and/or spermatogenesis;
- wherein said donor animals are of a different species than the recipient animal.
6 more elements
Dependencies are inferred from the claim text.