Method and means for producing high titer, safe, recombinant lentivirus vectors
AssigneeCell Genesys, Inc.
InventorsLuigi Naldini, Thomas Dull, Deborah A. Farson, Rochelle Witt
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Abstract
Lentiviral vectors modified at the 5′ LTR or both the 5′ and 3′ LTR's are useful in the production of recombinant lentivirus vectors. Such vectors can be produced in the absence of a functional tat gene. Multiple transformation of the host cell with the vector carrying the transgene enhances virus production.
Claims14
A lentivirus transfer vector comprising a 5′ LTR and a 3′ LTR, each of which contains a U3 region and an endogenous R region, wherein a part or all of a regulatory element of the U3 region of the 5′ LTR is replaced by another regulatory element, operable in a mammalian cell, which is not endogenous to said lentivirus, and wherein one or more nucleotide bases of the U3 region of the 3′ LTR are deleted.
A lentivirus packaging plasmid lacking sequences upstream from gag endogenous to said lentivirus, lacking sequences downstream from env endogenous to said lentivirus, and lacking a functional tat gene.
Dependencies are inferred from the claim text.