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US 7,083,942 B2Granted
Alleles of the aceA gene from coryneform bacteria
AssigneeDegussa AG
InventorsBrigitte Bathe, Caroline Kreutzer, Stephan Hans
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Abstract
The invention relates to alleles of the aceA gene from coryneform bacteria coding for isocitrate lyases and processes for the fermentative production of L-lysine using bacteria that contain these alleles.
Claims10
A process for the production of L-lysine or feedstuffs additives containing L-lysine, comprising:
- a) fermenting coryneform bacteria in which alleles of the endogenous aceA gene are over-expressed under conditions that are suitable for the formation of the aceA gene product isocitrate lyase, and wherein said isocitrate lyase comprises the amino acid sequence of SEQ ID NO:2 in which the alanine residue at position 332 is replaced with threonine;
- and
- b) isolating said L-lysine or the feedstuffs additive containing L-lysine from the fermentation broth, optionally
- c) with constituents from the fermentation broth and/or biomass (>0 up to 100%).
1 more element
A process for the production of L-lysine or feedstuffs additives containing L-lysine, comprising:
- a) fermenting coryneform bacteria that contain endogenous nucleotide sequences coding for the enzyme isocitrate lyase, wherein in the associated amino acid sequences the L-alanine at position 332 is replaced by L-threonine,
- b) optionally enriching the L-lysine in the fermentation broth,
- c) isolating the L-lysine or the feedstuffs additive containing L-lysine from the fermentation broth, optionally
- d) with constituents from the fermentation broth and/or from the biomass (>0 up to 100%).
1 more element
Dependencies are inferred from the claim text.