# US 7,078,587 B1 · Tapetum-specific promoters

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,078,587 B1
- Document ID: US7078587B1
- Status: Granted
- Kind: B1
- Application number: 09/959,856
- Filed: 2000-05-10
- Granted: 2006-07-18
- Earliest claimed: 1999-05-10
- Assignees: Biogemma UK Limited
- Inventors: Wyatt Paul; Roderick John Scott; Diane Hird; Rachel Hodge
- Web page: https://patentgrep.com/patent/US7078587B1

## CPC

- C07K 14/415: from plants
- C12N 15/8231: {Male-specific, e.g. anther, tapetum, pollen}
- C12N 15/8289: {Male sterility}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to nucleic acid sequences encoding tapetum specific promoters, for use in Artificial Male Sterility systems in plants. In particular, the promoters may be the pMAC2 promoter; the pMAC20 promoter; or promoter sequences which naturally controls the expression of a coding sequence substantially homologous to the MAC2 or MAC20 coding sequences. Also provided are regulatory elements of the promoters; plant cells and plants transformed with the promoter sequences.

## Claims

### Claim 1

A recombinant or isolated nucleic acid molecule comprising a pMAC2 promoter sequence comprising nucleotides 35 to 1258 of SEQ ID NO:19.

### Claim 2 (depends on 1)

A nucleic acid molecule as claimed in claim 1 which is a DNA molecule.

### Claim 3 (depends on 1)

A nucleic acid molecule as claimed in claim 1 which further comprises a nucleic acid, which when expressed results in male sterility in a plant.

### Claim 4 (depends on 3)

A nucleic acid molecule as claimed in claim 3 wherein the male sterility nucleic acid codes for a lytic enzyme.

### Claim 5 (depends on 4)

A nucleic acid molecule as claimed in claim 4 wherein the lytic enzyme is glucanase or barnase.

### Claim 6 (depends on 5)

A nucleic acid as claimed in claim 5 wherein the glucanase is PR-glucanase.

### Claim 7 (depends on 1)

A vector comprising a nucleic acid molecule as defined in claim 1.

### Claim 8 (depends on 7)

A host cell transformed with a vector as defined in claim 7.

### Claim 9 (depends on 8)

A host cell as claimed in claim 8 which is a plant cell.

### Claim 10 (depends on 9)

A host cell as claimed in claim 9 which is a monocot plant cell.

### Claim 11 (depends on 9)

A plant comprising one or more plant cells as defined in claim 9.

### Claim 12 (depends on 1)

A method of transforming a host cell comprising introducing the nucleic acid molecule as defined in claim 1 into the host cell with a particle gun.

### Claim 13 (depends on 3)

A method of producing a male sterile plant comprising:
(i) introducing the nucleic acid molecule as defined in claim 3 into a plant cell with a particle gun; and
(ii) generating said male sterile plant from the transformed plant cell.

### Claim 14 (depends on 13)

The method according to claim 13, wherein the male sterile plant is a monocot.