Gene encoding a nonribosomal peptide synthetase for the production of ramoplanin
AssigneeEcopia BioSciences, Inc.
InventorsChris M. Farnet, Emmanuel Zazopoulos, Alfredo Staffa
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Abstract
The present invention relates to an isolated genetic sequence encoding a nonribosomal peptide synthetase (NRPS) protein which directs the biosynthesis of the antibiotic ramoplanin in microorganisms such as Actinoplanes sp. The isolated gene sequence serves as a substrate for bioengineering of antibiotic structures.
Claims25
An isolated nucleic acid comprising a polynucleotide which encodes a domain of a ramoplanin nonribosomal peptide synthetase, wherein said ramoplanin nonribosomal peptide synthetase comprises the amino acid sequence of SEQ ID NO:
- 15 and wherein said domain is a condensation domain, an adenylation domain, a thiolation domain, or a thioesterase domain.
An isolated nucleic acid comprising a polynucleotide which encodes a module of a nonribosomal peptide synthetase comprising a domain of the ramoplanin nonribosomal peptide synthetase of SEQ ID NO:
- 15 wherein said domain is a condensation domain, an adenylation domain, a thiolation domain, or a thioesterase domain.
Dependencies are inferred from the claim text.