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AssigneesKirin Beer Kabushiki Kaisha, Hematech, LLC
InventorsJames M. Robl, Richard A. Goldsby, Stacy E. Ferguson, Yoshimi Kuroiwa, Kazuma Tomizuka, Isao Ishida, Barbara A. Osborne
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The present invention relates to the production of a transgenic bovine which comprises a genetic modification that results in inactivation and loss of expression of its endogenous antibodies, and the expression of xenogenous antibodies, preferably human antibodies. This is effected by inactivation of the IgM heavy chain expression and, optionally, by inactivation of the Ig light chain expression, and by the further introduction of an artificial chromosome which results in the expression of non-bovine antibodies, preferably human antibodies.
A transgenic bovine whose cells comprise one or more artificial chromosomes, each artificial chromosome comprising one or more human immunoglobulin heavy or light chain loci that undergo rearrangement and are expressed in B-cells to produce a human immunoglobulin in response to exposure to one or more antigens.
An isolated transgenic bovine B-cell comprising one or more artificial chromosomes, each artificial chromosome comprising one or more human immunoglobulin heavy or light chain loci that undergo rearrangement and are expressed to produce a human immunoglobulin in response to exposure to one or more antigens.
A method of producing human antibodies, said method comprising the steps of:
A method of producing human antibodies to one or more antigens, said method comprising recovering human antibodies from a transgenic bovine whose cells comprise one or more artificial chromosomes, each artificial chromosome comprising one or more human immunoglobulin heavy or light chain loci that undergo rearrangement and are expressed in B-cells, resulting in production of human antibodies against said one or more antigens.
Dependencies are inferred from the claim text.