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US 7,074,894 B2Granted
Antigen composition against mycoplasma
AssigneeThe University of Melbourne
InventorsJohn Walker, Rogan Lee, Stephen William Doughty
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AssigneeThe University of Melbourne
InventorsJohn Walker, Rogan Lee, Stephen William Doughty
Estimating the term…
An isolated antigen against a Mycoplasma, prepared by a method including providing a sample of a Mycoplasma and an antibody probe, probing the Mycoplasma sample with the antibody probe to detect at least one antigen, and isolating the antigen detected. The antibody probe includes at least one antibody against the Mycoplasma that is produced by a method including (a) providing a biological sample taken a short time after an immune animal has been challenged with a Mycoplasma or Mycoplasma extract taken from the infection site or an area of a lesion or an area close to the infection site or lesion; (b) isolating cells from the biological sample; (c) culturing the cells in vitro in a suitable culture medium; and (d) harvesting antibodies produced from the cell.
An isolated antigen prepared by a method comprising:
An isolated antigen comprising a molecular structure that is identifiable with an antibody probe produced by harvesting an antibody from antibody producing cells of a mammal that are at or close to an infection or lesion site within 2 to 5 days after said mammal is challenged by infection with Mycoplasma hyopneumoniae at said infection or lesion site, said molecular structure being a native Mycoplasma hyopneumoniae antigen having an approximate molecular weight in kilodaltons (kD) of between 110–114, 90–94, 72–75, 52–54 or 46–48, or being a mutant, derivative or fragment of the native antigen that stimulates production of the antibody in the antibody producing cells, wherein if the molecular structure is the native antigen having the molecular weight between 72–75 kD, the molecular structure contains an N-terminal amino acid sequence comprising SEQ ID NO:12, and wherein if the molecular structure has a molecular weight between 46–48 kD, the molecular structure has an N-terminal amino acid sequence comprising SEQ ID NO:3.
A method for preparing a synthetic antigenic polypeptide against Mycoplasma, which method comprises
An amino acid sequence encoded by a SEQ ID NO:1.
An amino acid sequence consisting of SEQ ID NO:2.
Dependencies are inferred from the claim text.