Bioproduction of astaxanthin using mutant carotenoid ketolase and carotenoid hydroxylase genes
AssigneeE. I. Du Pont de Nemours and Company
InventorsXiao-Song Tang, Qiong Cheng, Joanne Y. Shyr, Luan Tao
Life of this patent
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Abstract
Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts.
Claims9
An nucleic acid molecule encoding at least one carotenoid ketolase and at least one carotenoid hydroxylase, said nucleic acid molecule comprising:
- a) a nucleic acid fragment encoding a carotenoid ketolase having an amino acid sequence selected from the group consisting of SEQ ID NO:
- 17 and SEQ ID NO:
- 22;
- and
- b) a isolated nucleic acid fragment encoding a carotenoid hydroxylase having an amino acid sequence selected from the group consisting of SEQ ID NO:
- 19 and SEQ ID NO:
- 24;
- or
- an isolated nucleic acid molecule completely complementary to the nucleic acid molecule comprising (a) and (b).
An isolated nucleic acid molecule encoding at least one carotenoid ketolase and at least one carotenoid hydroxylase, said nucleic acid molecule comprising:
- a) a nucleic acid fragment encoding a carotenoid ketolase having a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 16 and SEQ ID NO:
- 21;
- and
- b) a nucleic acid fragment encoding a carotenoid hydroxylase having a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 18 and SEQ ID NO:
- 23;
- or
- an isolated nucleic acid molecule completely complementary to the nucleic acid molecule comprising (a) and (b).
An isolated nucleic acid molecule encoding a carotenoid ketolase and a carotenoid hydroxylase, said isolated nucleic acid molecule comprising:
- a) a nucleic acid fragment encoding a carotenoid ketolase having the amino acid sequence as represented by SEQ ID NO:
- 17 and
- a nucleic acid fragment encoding a carotenoid hydroxylase enzyme having the amino acid sequence as represented by SEQ ID NO:
- 19;
- or
- (b) an isolated nucleic acid molecule completely complementary to the nucleic acid fragment of (a).
An isolated nucleic acid molecule encoding a carotenoid ketolase and a carotenoid hydroxylase, said isolated nucleic acid molecule comprising:
- a) a nucleic acid fragment encoding a carotenoid ketolase having the amino acid sequence as represented by SEQ ID NO:
- 21;
- and
- a nucleic acid fragment encoding a carotenoid hydroxylase enzyme having the amino acid sequence as represented by SEQ ID NO:
- 23;
- or
- b) an isolated nucleic acid molecule completely complementary to the nucleic acid fragment of (a).
Dependencies are inferred from the claim text.