# US 7,071,397 B2 · Wheat variety XW01G

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- Number: US 7,071,397 B2
- Document ID: US7071397B2
- Status: Granted
- Kind: B2
- Application number: 10/852,736
- Filed: 2004-05-24
- Published: 2004-10-21
- Granted: 2006-07-04
- Earliest claimed: 2004-05-24
- Assignees: Pioneer Hi-Bred International, Inc.
- Inventors: Kyle Jay Lively; Robert Lewis Clarkson; William Joseph Laskar; Gregory Charles Marshall
- Web page: https://patentgrep.com/patent/US7071397B2

## CPC

- A01H 6/4678: {Triticum sp. [wheat]}
- A01H 5/04: Stems
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A wheat variety designated XW01G, the plants and seeds of wheat variety XW01G, methods for producing a wheat plant produced by crossing the variety XW01G with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety XW01G with another wheat line or plant, and the creation of variants by mutagenesis or transformation of variety XW01G. This invention also relates to methods for producing other wheat varieties or breeding lines derived from wheat variety XW01G and to wheat varieties or breeding lines produced by those methods.

## Claims

### Claim 1

A seed of wheat variety designated XW01G, representative seed of said variety having been deposited under ATCC Accession No. PTA-6803.

### Claim 2 (depends on 1)

A wheat plant, or part thereof, produced by growing the seed of claim 1.

### Claim 3 (depends on 2)

A tissue culture of regenerable cells produced from the plant of claim 2.

### Claim 4 (depends on 3)

A protoplast produced from the tissue culture of claim 3.

### Claim 5 (depends on 2)

The tissue culture of claim 2, wherein cells of the tissue culture are from a tissue selected from the group consisting of kernel, head, stem, leaves, root, root tip, pollen, ovule, embryo and flower.

### Claim 6 (depends on 3)

A wheat plant regenerated from the tissue culture of claim 3, said plant having all the morphological and physiological characteristics of wheat variety XW01G, representative seed of said wheat variety deposited under ATCC Accession No. PTA-6803.

### Claim 7 (depends on 2)

A method for producing an F1 wheat seed, comprising crossing the plant of claim 2 with a different wheat plant and harvesting the resulting F1 wheat seed.

### Claim 8 (depends on 2)

A method of producing a male sterile wheat plant, wherein the method comprises transforming the wheat plant of claim 2 with a nucleic acid molecule that confers male sterility.

### Claim 9 (depends on 8)

A male sterile wheat plant produced by the method of claim 8.

### Claim 10 (depends on 2)

A method of producing an herbicide resistant wheat plant, wherein the method comprises transforming the wheat plant of claim 2 with a transgene that confers herbicide resistance.

### Claim 11 (depends on 10)

An herbicide resistant wheat plant produced by the method of claim 10.

### Claim 12 (depends on 11)

The wheat plant of claim 11, wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

### Claim 13 (depends on 2)

A method of producing an insect resistant wheat plant, wherein the method comprises transforming the wheat plant of claim 2 with a transgene that confers insect resistance.

### Claim 14 (depends on 13)

An insect resistant wheat plant produced by the method of claim 13.

### Claim 15 (depends on 14)

The wheat plant of claim 14, wherein the transgene encodes a Bacillus thuringiensis endotoxin.

### Claim 16 (depends on 2)

A method of producing a disease resistant wheat plant, wherein the method comprises transforming the wheat plant of claim 2 with a transgene that confers disease resistance.

### Claim 17 (depends on 16)

A disease resistant wheat plant produced by the method of claim 16.

### Claim 18 (depends on 17)

The wheat plant of claim 17, wherein the transgene confers resistance to Fusarium graminearum through the detoxification of deoxynivalenol.

### Claim 19 (depends on 2)

A method of producing a wheat plant with decreased phytate content, wherein the method comprises transforming the wheat plant of claim 2 with a transgene encoding phytase.

### Claim 20 (depends on 19)

A wheat plant with decreased phytate content produced by the method of claim 19.

### Claim 21 (depends on 2)

A method of producing a wheat plant with modified fatty acid metabolism, modified protein metabolism or modified carbohydrate metabolism, wherein the method comprises transforming the wheat plant of claim 2 with a transgene encoding a polypeptide selected from the group consisting of modified glutenins, gliadins, fructosyltransferase, levasucrase, alpha-amylase, invertase and starch branching enzyme or encoding an antisense stearoyl-ACP-desaturase.

### Claim 22 (depends on 21)

A wheat plant having modified fatty acid metabolism, modified protein metabolism or modified carbohydrate metabolism, wherein the wheat plant is produced by the method of claim 21.

### Claim 23 (depends on 22)

The wheat plant of claim 22 wherein the transgene confers a trait selected from the group consisting of waxy starch and increased amylose starch.

### Claim 24

A wheat plant, or part thereof, having all the physiological and morphological characteristics of the variety XW01G, representative seed of such line having been deposited under ATCC Accession No. PTA-6803.

### Claim 25 (depends on 2)

A method of producing a wheat plant with increased tolerance to water stress, wherein the method comprises transforming the wheat plant of claim 2 with a transgene encoding mannitol-1-phosphate dehydrogenase or late embryogenesis abundant (LEA) proteins.

### Claim 26 (depends on 25)

A wheat plant with increased tolerance to water stress produced by the method of claim 25.

### Claim 27

A method of introducing a desired trait into wheat variety XW01G, wherein the method comprises:
(a) crossing XW01G plants grown from XW01G seed, representative seed of which has been deposited under ATCC Accession No. PTA-6803, with plants of another wheat line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, disease resistance and waxy starch;
(b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants;
(c) crossing the selected progeny plants with the XW01G plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of wheat variety XW01G listed in Table 1 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of wheat variety XW01G listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

### Claim 28 (depends on 27)

A plant produced by the method of claim 27, wherein the plant has the desired trait and all of the physiological and morphological characteristics of wheat variety XW01G listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

### Claim 29 (depends on 28)

The plant of claim 28, or a part of the plant, wherein the desired trait is herbicide resistance and the resistance is conferred to a herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

### Claim 30 (depends on 28)

The plant of claim 28, or a part of the plant, wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.

### Claim 31 (depends on 28)

The plant of claim 28, or a part of the plant, wherein the desired trait is disease resistance to fusarium graminearum and the disease resistance is conferred through the detoxification of deoxynivalenol.

### Claim 32 (depends on 28)

The plant of claim 28, or a part of the plant, wherein the desired trait is male sterility and the trait is conferred by a cytoplasmic nucleic acid molecule that confers male sterility.

### Claim 33

A method of modifying fatty acid metabolism, phytic acid metabolism, carbohydrate metabolism, waxy starch content, gluten content, or water stress tolerance in wheat variety XW01G, wherein the method comprises:
(a) crossing XW01G plants grown from XW01G seed, representative seed of which has been deposited under ATCC Accession No. PTA-6803, with plants of another wheat variety that comprises a nucleic acid molecule encoding a polypeptide selected from the group consisting of phytase, fructosyltransferase, levansucrase, alpha-amylase, invertase, starch branching enzyme, glutenin, gliadin, mannitol-1-phosphate dehydrogenase and a late embryogenesis abundant (LEA) protein or encoding an antisense stearoyl-ACP destaturase;
(b) selecting F1 progeny plants that have said nucleic acid molecule to produce selected F1 progeny plants;
(c) crossing the selected progeny plants with the XW01G plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have said nucleic acid molecule and physiological and morphological characteristics of wheat variety XW01G listed in Table 1 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise said nucleic acid molecule and have all of the physiological and morphological characteristics of wheat variety XW01G listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

### Claim 34 (depends on 33)

A plant produced by the method of claim 33, wherein the plant comprises the nucleic acid molecule and has all of the physiological and morphological characteristics of wheat variety XW01G listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.