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US 7,071,381 B1Granted
Plant vitamin e biosynthetic enzymes
AssigneeE. I. Du Pont de Nemours and Company
InventorSean J. Coughlan
Life of this patent
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a vitamin E biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the vitamin E biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the vitamin E biosynthetic enzyme in a transformed host cell.
Claims13
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a gamma tocopherol methyltransferase having an amino acid sequence of at least 90% sequence identity, based on the Clustal method of alignment with pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, when compared to SEQ ID NO:28;
- or
- (b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence have the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.