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US 7,071,372 B2Granted
Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
AssigneesUniversity of Connecticut, The Governor of Kagoshima Prefecture
InventorsXiangzhong Yang, Chikara Kubota
Life of this patent
Estimating the term…
Abstract
An improved method of nuclear transfer employing long-term cultured somatic cells as the donor cells and enucleated oocytes as the recipient cells to produce dividing cybrids. Such cybrids are useful for developing viable animals clones when nurtured in a suitable host environment.
Claims13
A method of improving pregnancy rates in a female bovine, comprising:
- culturing adult bovine fibroblast donor cells in serum starved media;
- passaging the cells for at least 10 and up to 15 passages;
- nuclear transferring the donor cells into enucleated recipient bovine oocytes to form a cybrid;
- culturing the cybrid;
- and
- transferring the cybrid into a recipient female bovine.
3 more elements
A method of improving pregnancy rates in a female bovine, comprising:
- culturing adult bovine fibroblast donor cells in serum starved media to obtain 14, 20, or 30 doublings in cell number;
- nuclear transferring the donor cells into enucleated recipient bovine oocytes to form a cybrid;
- culturing the cybrid;
- and
- transferring the cybrid into a recipient female bovine.
2 more elements
Dependencies are inferred from the claim text.