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US 7,071,307 B2Granted
Nucleic acids and proteins with thioredoxin reductase activity
AssigneesSyngenta Participations AG, Xencor, Inc.
InventorsBipin K. Dalmia, Steven P. Briggs, Greg del Val, John R. Desjarlais, Peter Heifetz, Peter Luginbuhl, Umesh Muchhal
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Abstract
The present invention relates to the use of a variety of methods for generating functional thioredoxin reductase variants in which at least one physical, chemical or biological property of the variant is altered in a specific and desired manner when compared to the wild-type protein.
Claims11
A variant thioredoxin reductase (TR) protein with altered cofactor specificity as compared to a naturally occurring thioredoxin reductase protein comprising an isolated polypeptide molecule having the formula:
- S1-A1-A2-S2-A3-A4-A5-S3-A6-S4
- wherein
- a) S1 comprises SEQ ID NO:6;
- b) S2 comprises SEQ ID NO:13;
- c) S3 comprises SEQ ID NO:20;
- d) S4 comprises SEQ ID NO:27;
- e) A1 is the amino acid serine;
- f) A2 is the amino acid is the amino acid alanine;
- g) A3 is the amino acid is the amino acid histidine;
- h) A4 is an amino acid moiety selected from the group consisting of arginine and tryptophan;
- i) A5 is an amino acid moiety selected from the group consisting of arginine, valine, leucine, isoleucine, methionine, phenylalanine, and tyrosine;
- j) A6 is an amino acid moiety selected from the group consisting of arginine, glycine, asparagine, glutamine, serine, and threonine, wherein only zero, one, or two of A4, A5 or A6 are arginine.
9 more elements
Dependencies are inferred from the claim text.