Lentiviral packaging constructs
AssigneeNovartis AG
InventorsMichael Kaleko, Tianci Luo, Ivan Plavec, Janet Lynn Douglas
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Abstract
The present invention provides novel lentiviral packaging constructs that are useful for the establishment of stable packaging cell lines and producer cell lines. In particular, the present invention provides novel packaging cell lines that are capable of constitutively expressing high levels of lentiviral proteins.
Claims24
A lentiviral packaging construct for use in generating lentiviral vectors comprising a sequence encoding a lentiviral protease gene and a deletion in a lentiviral packaging signal sequence, wherein said sequence encoding said protease includes a substitution wherein the T in the DTGAD (residues 25–29 of SEQ ID NO:1) motif is replaced with an S.
Dependencies are inferred from the claim text.